| Primer | Species | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 161 | Brachyspira pilosicoli | GGCGAGAGATGTCTTACGGC | ACCAGCTACACGGTTAGCC | 60.60 | 59.40 | 192 | 69 |
100.00%
|
100.00%
|
| Primer 162 | Brachyspira pilosicoli | GCAAGAGCAGGTGCAACT | TAGGGTGGCGAACACTTGC | 58.24 | 60.30 | 151 | 69 |
100.00%
|
100.00%
|
| Primer 163 | Brachyspira pilosicoli | GGTGGCAAGGCTTCTCACT | TGTCCCCATCAAGCCTCAC | 59.93 | 59.31 | 158 | 69 |
100.00%
|
50.00%
|
| Primer 164 | Brachyspira pilosicoli | GGTGGCAAGGCTTCTCACT | CCCCATCAAGCCTCACACT | 59.93 | 59.31 | 155 | 69 |
100.00%
|
50.00%
|
| Primer 165 | Brachyspira pilosicoli | AGGTGGCAAGGCTTCTCAC | CCCCATCAAGCCTCACACT | 59.93 | 59.31 | 156 | 69 |
100.00%
|
50.00%
|
| Primer 166 | Brachyspira pilosicoli | TACACCTGCGGTTGTGCA | CGCTCACCAAAGCAGGAAG | 59.49 | 59.13 | 266 | 69 |
100.00%
|
100.00%
|
| Primer 167 | Brachyspira pilosicoli | ACGCAAATTCGCTGATGGTG | GAACGCCAGAAGCTTGCA | 59.83 | 58.66 | 272 | 69 |
100.00%
|
100.00%
|
| Primer 168 | Brachyspira pilosicoli | AAGGCTGCTGATGAGGCTC | TTCAAAGCCCTCATCATAGCC | 59.78 | 58.06 | 234 | 69 |
100.00%
|
100.00%
|
| Primer 169 | Brachyspira pilosicoli | ACAGGCTGAAGTTGCGGAG | GCCCTTCTCTGTCTAGTAGCC | 60.30 | 59.32 | 267 | 69 |
100.00%
|
100.00%
|
| Primer 170 | Brachyspira pilosicoli | ATTGCACACTTTATGACGGCA | AGCTCCTCCCAAAATAGCTCC | 58.84 | 59.51 | 170 | 69 |
100.00%
|
100.00%
|